dr yi zhang (Addgene inc)
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Dr Yi Zhang, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yi+zhang/pcDNA-Flag-Tet3+(Plasmid+%2360940)/pmc12310658-89-11-15
Average 91 stars, based on 5 article reviews
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DNA Methylation Assay:Article Title: Cell-specific exon methylation and CTCF binding in neurons regulate calcium ion channel splicing and function Article Snippet: .. Genomic DNA methylation and hydroxyl-methylation ELISAs Genomic DNA methylation and hydroxyl-methylation ELISAs gDNA was extracted from F11 cells using QIAamp DNA Mini Kit (QIAGEN Cat# 51304) and global DNA methylation (5-mC) and hydroxyl-methylation (5-hmC) levels were measured by ELISA assays (MethylFlash Methylated DNA 5-mC Quantification Kit - EpiGentek Cat# P-1030; MethylFlash Global DNA Hydroxymethylation 5- hmC ELISA Easy Kit - EpiGentek Cat# P-1032) according to manufacturer’s protocols. cDNA plasmids and siRNAs cDNA plasmids and siRNAs cDNA plasmid expression vectors are listed in Key Resources Table. pcDNA3-Tet1 (Addgene plasmid # 60938; http://n2t.net/addgene:60938; RRID:Addgene_60938), pcDNA3-Tet2 (Addgene plasmid # 60939; http://n2t.net/addgene:60939; RRID:Addgene_60939), and pcDNA-Flag-Tet3 were gifts from Enzyme-linked Immunosorbent Assay:Article Title: Cell-specific exon methylation and CTCF binding in neurons regulate calcium ion channel splicing and function Article Snippet: .. Genomic DNA methylation and hydroxyl-methylation ELISAs Genomic DNA methylation and hydroxyl-methylation ELISAs gDNA was extracted from F11 cells using QIAamp DNA Mini Kit (QIAGEN Cat# 51304) and global DNA methylation (5-mC) and hydroxyl-methylation (5-hmC) levels were measured by ELISA assays (MethylFlash Methylated DNA 5-mC Quantification Kit - EpiGentek Cat# P-1030; MethylFlash Global DNA Hydroxymethylation 5- hmC ELISA Easy Kit - EpiGentek Cat# P-1032) according to manufacturer’s protocols. cDNA plasmids and siRNAs cDNA plasmids and siRNAs cDNA plasmid expression vectors are listed in Key Resources Table. pcDNA3-Tet1 (Addgene plasmid # 60938; http://n2t.net/addgene:60938; RRID:Addgene_60938), pcDNA3-Tet2 (Addgene plasmid # 60939; http://n2t.net/addgene:60939; RRID:Addgene_60939), and pcDNA-Flag-Tet3 were gifts from Methylation:Article Title: Cell-specific exon methylation and CTCF binding in neurons regulate calcium ion channel splicing and function Article Snippet: .. Genomic DNA methylation and hydroxyl-methylation ELISAs Genomic DNA methylation and hydroxyl-methylation ELISAs gDNA was extracted from F11 cells using QIAamp DNA Mini Kit (QIAGEN Cat# 51304) and global DNA methylation (5-mC) and hydroxyl-methylation (5-hmC) levels were measured by ELISA assays (MethylFlash Methylated DNA 5-mC Quantification Kit - EpiGentek Cat# P-1030; MethylFlash Global DNA Hydroxymethylation 5- hmC ELISA Easy Kit - EpiGentek Cat# P-1032) according to manufacturer’s protocols. cDNA plasmids and siRNAs cDNA plasmids and siRNAs cDNA plasmid expression vectors are listed in Key Resources Table. pcDNA3-Tet1 (Addgene plasmid # 60938; http://n2t.net/addgene:60938; RRID:Addgene_60938), pcDNA3-Tet2 (Addgene plasmid # 60939; http://n2t.net/addgene:60939; RRID:Addgene_60939), and pcDNA-Flag-Tet3 were gifts from Plasmid Preparation:Article Title: Cell-specific exon methylation and CTCF binding in neurons regulate calcium ion channel splicing and function Article Snippet: .. Genomic DNA methylation and hydroxyl-methylation ELISAs Genomic DNA methylation and hydroxyl-methylation ELISAs gDNA was extracted from F11 cells using QIAamp DNA Mini Kit (QIAGEN Cat# 51304) and global DNA methylation (5-mC) and hydroxyl-methylation (5-hmC) levels were measured by ELISA assays (MethylFlash Methylated DNA 5-mC Quantification Kit - EpiGentek Cat# P-1030; MethylFlash Global DNA Hydroxymethylation 5- hmC ELISA Easy Kit - EpiGentek Cat# P-1032) according to manufacturer’s protocols. cDNA plasmids and siRNAs cDNA plasmids and siRNAs cDNA plasmid expression vectors are listed in Key Resources Table. pcDNA3-Tet1 (Addgene plasmid # 60938; http://n2t.net/addgene:60938; RRID:Addgene_60938), pcDNA3-Tet2 (Addgene plasmid # 60939; http://n2t.net/addgene:60939; RRID:Addgene_60939), and pcDNA-Flag-Tet3 were gifts from Article Title: B-type Plexins promote the GTPase activity of Ran to affect androgen receptor nuclear translocation in prostate cancer. Article Snippet: .. Expression vectors and site-directed mutagenesis pcDNA3-PlexinB1 full-length-myc (PlexinB1-myc), pcDNA3-Plexin B1 cytoplasmic domain-Myc (cytoPlexinB1-myc), PlexinB1(RA)-myc (R1677A, R1678A, R1984A) and cytoPlexinB1(RA)-myc were kind gifts of Dr I Oinuma (Kyoto, Japan). pmCherry-C1-RanQ69L was a gift from Jay Brenman (Addgene plasmid # 30309; http://n2t.net/addgene:30309; RRID:Addgene_30309), Cancer Gene Therapy (2023) 30:1513 – 1523 pcDNA-Ran-T24N-Mrfp1-polyA from Article Title: iPS cell generation-associated point mutations include many C > T substitutions via different cytosine modification mechanisms. Article Snippet: All animal procedures were approved by the Ethics Committee of the National Institutes for Quantum Science and Technology. .. Plasmid Construction and mouse iPSCs generation pMXs- Oct3/4, -Sox2, -Klf4 and -c-Myc were gifts from Shinya Yamanaka (Addgene plasmid # 13366, #13367, #13370 and #13375)1. pcDNA3-Tet1 and pcDNA3-Tet2 were gifts from Expressing:Article Title: Cell-specific exon methylation and CTCF binding in neurons regulate calcium ion channel splicing and function Article Snippet: .. Genomic DNA methylation and hydroxyl-methylation ELISAs Genomic DNA methylation and hydroxyl-methylation ELISAs gDNA was extracted from F11 cells using QIAamp DNA Mini Kit (QIAGEN Cat# 51304) and global DNA methylation (5-mC) and hydroxyl-methylation (5-hmC) levels were measured by ELISA assays (MethylFlash Methylated DNA 5-mC Quantification Kit - EpiGentek Cat# P-1030; MethylFlash Global DNA Hydroxymethylation 5- hmC ELISA Easy Kit - EpiGentek Cat# P-1032) according to manufacturer’s protocols. cDNA plasmids and siRNAs cDNA plasmids and siRNAs cDNA plasmid expression vectors are listed in Key Resources Table. pcDNA3-Tet1 (Addgene plasmid # 60938; http://n2t.net/addgene:60938; RRID:Addgene_60938), pcDNA3-Tet2 (Addgene plasmid # 60939; http://n2t.net/addgene:60939; RRID:Addgene_60939), and pcDNA-Flag-Tet3 were gifts from Article Title: B-type Plexins promote the GTPase activity of Ran to affect androgen receptor nuclear translocation in prostate cancer. Article Snippet: .. Expression vectors and site-directed mutagenesis pcDNA3-PlexinB1 full-length-myc (PlexinB1-myc), pcDNA3-Plexin B1 cytoplasmic domain-Myc (cytoPlexinB1-myc), PlexinB1(RA)-myc (R1677A, R1678A, R1984A) and cytoPlexinB1(RA)-myc were kind gifts of Dr I Oinuma (Kyoto, Japan). pmCherry-C1-RanQ69L was a gift from Jay Brenman (Addgene plasmid # 30309; http://n2t.net/addgene:30309; RRID:Addgene_30309), Cancer Gene Therapy (2023) 30:1513 – 1523 pcDNA-Ran-T24N-Mrfp1-polyA from Mutagenesis:Article Title: B-type Plexins promote the GTPase activity of Ran to affect androgen receptor nuclear translocation in prostate cancer. Article Snippet: .. Expression vectors and site-directed mutagenesis pcDNA3-PlexinB1 full-length-myc (PlexinB1-myc), pcDNA3-Plexin B1 cytoplasmic domain-Myc (cytoPlexinB1-myc), PlexinB1(RA)-myc (R1677A, R1678A, R1984A) and cytoPlexinB1(RA)-myc were kind gifts of Dr I Oinuma (Kyoto, Japan). pmCherry-C1-RanQ69L was a gift from Jay Brenman (Addgene plasmid # 30309; http://n2t.net/addgene:30309; RRID:Addgene_30309), Cancer Gene Therapy (2023) 30:1513 – 1523 pcDNA-Ran-T24N-Mrfp1-polyA from Generated:Article Title: B-type Plexins promote the GTPase activity of Ran to affect androgen receptor nuclear translocation in prostate cancer. Article Snippet: .. Expression vectors and site-directed mutagenesis pcDNA3-PlexinB1 full-length-myc (PlexinB1-myc), pcDNA3-Plexin B1 cytoplasmic domain-Myc (cytoPlexinB1-myc), PlexinB1(RA)-myc (R1677A, R1678A, R1984A) and cytoPlexinB1(RA)-myc were kind gifts of Dr I Oinuma (Kyoto, Japan). pmCherry-C1-RanQ69L was a gift from Jay Brenman (Addgene plasmid # 30309; http://n2t.net/addgene:30309; RRID:Addgene_30309), Cancer Gene Therapy (2023) 30:1513 – 1523 pcDNA-Ran-T24N-Mrfp1-polyA from Subcloning:Article Title: B-type Plexins promote the GTPase activity of Ran to affect androgen receptor nuclear translocation in prostate cancer. Article Snippet: .. Expression vectors and site-directed mutagenesis pcDNA3-PlexinB1 full-length-myc (PlexinB1-myc), pcDNA3-Plexin B1 cytoplasmic domain-Myc (cytoPlexinB1-myc), PlexinB1(RA)-myc (R1677A, R1678A, R1984A) and cytoPlexinB1(RA)-myc were kind gifts of Dr I Oinuma (Kyoto, Japan). pmCherry-C1-RanQ69L was a gift from Jay Brenman (Addgene plasmid # 30309; http://n2t.net/addgene:30309; RRID:Addgene_30309), Cancer Gene Therapy (2023) 30:1513 – 1523 pcDNA-Ran-T24N-Mrfp1-polyA from |
